清热中药对ApoE基因缺陷小鼠动脉粥样硬化斑块的影响

摘要

Objective :To observe effects of paniculta,scutellaria, anemarrhena,cortex moutan and artemisia on atherosclerotic plaque in mice with apolipoprotein E deficient. Methods:Model mice were divided randomly into the control group,the paniculta group,the scutellaria group,the anemarrhena group,the cortex moutan group,the aremisia group and the simvastatin group. After 13 weeks treatments,the lipid level and the hs-CRP content were detected. The aorta indices were observed by the pathological method including the plaque area,the vascular crosssectional area,the vascular cross-sectional area,the lipid central area and the minimum fiber cap thickness. These indices were used to evaluate the correction plate area and the percentage of lipid central area in the plaque area-Results:Compared with the control group, there were no statistical differences among above herb groups. However,the hs-CRP contents in these groups were decreased obviously. The regulate plaque areas in the paniculta group,the scutellaia group and the artemisia group were lower remarkably than it in the control group.The lipid central areas in the herb groups were lower than it in the control group. In the paniculta group,the scutellaria group and the atemisia group ,the percentages of lipid central area in the plaque area were lower significantly than it in the control group. The minimum fiber cap thicknesses of these herb groups were higer obviously than it in the control group.Conclusion:These clearing heat herbs may stabilize the atherosclerotic plaques in ApoE gene-deficient mice by interfering their structure.And it might be relative with their anti-inflammatory effects.%目的 观察穿心莲、黄芩、知母、牡丹皮和青蒿对ApoE基因缺陷小鼠动脉粥样硬化斑块的影响.方法 以ApoE基因缺陷小鼠制作动脉粥样硬化模型,随机分为对照组、穿心莲组、黄芩组、知母组、牡丹皮组、青蒿组及辛伐他汀组,治疗13周后测定血脂、超敏C反应蛋白(hs-CRP),并取主动脉进行病理学观察,测量斑块画积、血管横截面积、脂质中心面积、最小纤维帽厚度,计算校正斑块面积(斑块面积/血管横截面积)及脂质中心面积占斑块面积百分比.结果 与对照组比较,各清热中药组血脂无明显差异,但hs-CRP明显下降.黄芩组、知母组、青蒿组的校正斑块面积明显低于对照组,各中药组脂质中心面积明显低于对照组,穿心莲组、黄芩组、青蒿组的脂质中心面积在斑块中的百分比明显低于对照组;各中药组的最小纤维帽厚度明显高于对照组.结论 上述清热中药可能干预ApoE缺陷小鼠动脉粥样硬化斑块的形态结构,稳定斑块,其作用可能与抗炎有关.