参附注射液预处理改善大鼠急性脑梗死后氧化应激的实验研究

摘要

Objective:To observe the effect of different doses of Simvastatin on the inhibition of oxidative stress,and to explore the potential mechanism of different doses of Simvastatin in improving the oxidative stress in rats with cerebral infarction.Methods:70 male Wistar rats were randomly divided into the blank group,the sham operation group,the model group and the simvastatin groups(high,medium and low dose).In the model group and simvastatin groups,the cerebral infarction model was made with suture method;the sham operation group was only operated without modeling.Finally,the rats with successful operation or modeling were selected into the groups,10 rats in each group.Evans blue method was used to detect blood-brain barrier permeability;given different doses of simvastatin intervention,total superoxide dismutase(SOD) in rat serum was determined,as well as copper and zinc-superoxide dismutase (CuZn-SOD) activity,and blood hydrogen peroxide (H2O2) differences,and all of the data was compared with that of the blank group.Results:The content of Evans blue in the model group was significantly higher than that in the blank group and the sham operation group.Besides,SOD,CuZn-SOD and H2O2 expression in serum increased in the model group(P＜0.01).Evans blue in different doses of simvastatin intervention groups declined,compared with that in the model group (P＜ 0.01);SOD,CuZn-SOD and H2O2 expres sion decreased (P＜ 0.01),especially in high dose group.Conclusion:Simvastatin can decrease the expression of oxidative stress in patients with acute cerebral infarction,which may be one of the mechanisms to reduce the blood-brain barrier permeability,protect the nerve cells and restore the function of the nervous system,and has a certain correlation with the dose.%目的 观察不同剂量参附注射液抑制氧化应激的作用,探求不同剂量参附注射液改善大鼠脑梗死后氧化应激的潜在机制.方法 选取70只雄性Wistar大鼠,随机分为空白组、假手术组、模型组和参附注射液组(高、中、低剂量).除空白组外,模型组和参附注射液组采用线栓法制作大鼠脑梗死模型,假手术组仅进行手术不造模.最终选取手术和造模成功的大鼠人组,每组10只.采用伊文思蓝法检测血脑屏障通透性,给予不同剂量参附注射液干预,测定大鼠血清中总超氧化物歧化酶(SOD)、铜锌-超氧化物歧化酶(CuZn-SOD)活性,血液过氧化氢(H2O2)水平差异,并与空白组比较.结果 模型组组伊文思蓝含量明显高于空白组和假手术组,同时模型组血清中SOD、CuZn-SOD和H2O2表达升高(P＜0.01).不同剂量参附注射液干预组伊文思蓝均较模型组下降(P＜0.01),SOD、CuZn-SOD和H2O2表达下降(P＜ 0.01),尤其以高剂量参附注射液干预组更明显.结论 参附注射液减少急性脑梗死后氧化应激反应,可能通过降低血脑屏障通透性,保护神经细胞、恢复神经功能发挥作用,并呈剂量依赖.