参附注射液对脓毒症大鼠心血管功能和心肌氧化应激的改善作用

摘要

目的观察参附注射液对脓毒症大鼠心血管功能和心肌氧化应激的改善作用。方法采用结扎穿孔盲肠法制备腹腔感染脓毒症动物模型。将180只 SD 大鼠按随机数字表法分为假手术（Sham）组、脓毒症（CLP）组、参附注射液低剂量（LSF）组、参附注射液高剂量（HSF）组，每组各20只。记录手术后6、18 h 各组的平均动脉压（MAP）、心率（HR）、左室收缩压（LVDP）和左室最大收缩速率（+dP/dt）、左室舒张压（LVEDP）和左室最大舒张速率（-dP/dt）、心肌细胞凋亡发生率（AI）、心脏组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性、呼吸控制比（RCR）。结果与 Sham 组比较，CLP 组术后6h 时的 HR 显著升高（P＜0.05），但术后18h 时的HR 有所降低（P＜0.01）；术后6、18h 的 MAP、LVDP、+dP/dt 、LVEDP、-dP/dt、SOD、RCR 均显著下降（均 P＜0.01），AI、MDA 均显著升高（P＜0.05或0.01）。而与 CLP 组比较，LSF 组术后6h 的 MAP 和 HR、HSF 组术后6h 的 HR 差异均无统计学意义（均 P ＞0.05），但 HSF 组术后6h 的 MAP 及 LSF、HSF 组术后18h 的 HR、MAP 均明显升高（P＜0.05或0.01）；LSF 组和 HSF 组术后6、18h 的LVDP、+dP/dt、LVEDP、-dP/dt、SOD、RCR 均显著升高（P＜0.05或0.01）；LSF 组和 HSF 组术后6、18h 的 AI、MDA 均显著降低（P＜0.05或0.01）。结论参附注射液可改善脓毒症大鼠的左室收缩和舒张功能，减少心肌细胞凋亡和心肌组织 MDA 含量，增加 SOD活性，保护线粒体功能，且这些作用的强度与参附注射液药物剂量成正比。%Objective To investigate the effects of ShenFu Injection(SF) on cardiovascular function and myocardial oxida-tive stress in rats with sepsis. Methods A total of 80 Sprague-Dawley (SD) rats were subjected to sepsis by cecal ligation and puncture(CLP), and were randomly divided into four groups: sham operation group (n=20), sepsis group (n=20), low-SF group (n=20) and high-SF group(n=20). Mean arterial pressure (MAP), heart rate (HR), left ventricle developed pressure (LVDP) and maximal rate of the left ventricular pressure increase (+dP/dt), left ventricular end-diastolic pressure (LVEDP) and maximal rate of the left ventricular pressure decrease(-dP/dt), apoptosis incidence of myocardial cel s (AI), heart tissue malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and respiratory control ratio (RCR) of each group were recorded 6h and 18h after the surgery. Results Compared to Sham group, HR in CLP group increased significantly 6h after surgery (P<0.05), but de-creased significantly 18h after surgery (P<0.01). Compared with Sham group, MAP, LVDP, +dP/dt, LVEDP, -dP/dt , SOD and RCR in CLP group al decreased significantly 6h and 18h after surgery (P<0.01), AI and MDA increased significantly 6h and 18h after surgery (P<0.05 or 0.01). Compared with sepsis group, no significant differences were found in MAP and HR of LSF group 6h after surgery, and also in HR of HSF group 6h after surgery (P >0.05). Compared to sepsis group, MAP in HSF group 6h after surgery , HR and MAP in both LSF and HSF groups 18h after surgery al increased significantly (P<0.05 or 0.01). Compared to sepsis group, LVDP, +dP/dt, LVEDP, -dP/dt, SOD and RCR in both LSF and HSF groups increased significantly(P<0.05 or 0.01) 6h and 18h after surgery. Compared to sepsis group, AI and MDA in both LSF and HSF groups decreased significantly (P<0.05 or 0.01). Conclusion ShenFu Injection can improve heart function, decrease myocardial apoptosis, reduce myocardial MDA content, enhance SOD activity and protect mitochondrial function in septic rats. The effects are related to the dose of ShenFu In-jection.