肾脑复元汤对MCAO大鼠脑保护作用及cyt-C,Caspase-9,Caspase-3的影响

摘要

目的 探究肾脑复元汤对MCAO大鼠脑保护作用,探讨其脑保护机制与cyt-C,Caspase-9,Caspase-3表达的关系.方法 72只SD大鼠造模成功后随机分为假手术组、模型组、中药组,根据治疗后处死时间每组再随机分为3、7、14d 3个亚组,比较各组大鼠神经功能缺损评分、脑梗死体积,并采用Western blot法检测各组脑组织cyt-C,Caspase-9,Caspase-3的蛋白表达.结果 各时间点神经功能缺损评分比较显示,中药组较模型组有明显下降(P＜ 0.05或P＜0.01);脑梗死体积比较显示,中药组在各时间点脑梗死体积均明显小于模型组(P＜0.01);中药组3、7、14d脑组织cyt-C、Caspase-9、Caspase-3表达均低于模型组(P＜ 0.05或P＜0.01).结论 肾脑复元汤能促进MCAO大鼠神经功能恢复,减小脑梗死体积,其机制可能通过抑制线粒体cyt-C释放,减少Caspase-9、Caspase-3表达发挥对MCAO大鼠脑保护的作用.%Objective:To investigate the cerebral protection effect of Shennao Fuyuan decoction,find out its relation with the regulation of cyt-C,Caspase-9,and Caspase-3 expression.Methods:72 Sprague-Dawley (SD)rats were randomly divided into 3 groups:sham operation group,model group,traditional Chinese medicine group (TCM group).According to the executing time of 3,7,14 days,rats in each group were divided into three subgroups.Comparison of Neurological Severity Scores (NSS),volume of cerebral infarction among these groups was made.Western blot was used to explore the expression of cyt-C,Caspase-9,Caspase-3.Results:In comparison of NSS scores at every time point,TCM groups were lower than model groups,there were statistical differences between the two groups(P＜ 0.05 or P＜ 0.01).In comparison of infarct volume at every timne point,TCM groups were much lower than model groups;there were significant statistical differences between the two groups (P＜0.01).In comparison of protein expression of cyt-C,Caspase-9,Caspase-3 at every time point,TCM groups were lower than model groups,there were statistical differences between the two groups (P＜ 0.05 or P＜ 0.01).Conclusion:Shennao Fuyuan decoction can promote the recovery of neurological function;decrease the infarct volume in MCAO rats.This cerebral protection effect is probably related to restraining the release of cyt-C in mitochondria,and reducing the expression of Caspase-9,Caspase-3.