益气活血通络方对博莱霉素致肺纤维化大鼠TGF-β1/Smads言号通路的作用机制研究

摘要

Objective:To investigate the mechanism of Yiqi Huoxue Tongluo decoction on pulmonary fibrosis rats induced by bleomycin.Methods:60 rats were randomly divided into the blank group,the model group,hor mone group and TCM group.Except the blank group,the other three groups were established into the pulmonary fibrosis model induced by injecting with 5 mg/kg bleomycin.After modeling,the hormone group and TCM group were given corresponding drugs by garage;the blank group and the model group were treated with 0.9％ Nacl so lution orally,once a day.28 days later,the rats were killed and Masson staining was used to observe the pathological changes of lung tissue,weigh lung weight and calculate the lung coefficient and determination of hydroxyproline in lung tissue by Jamall and to detect the expression level of TGF-β1,Smad2 and Smad7 in rats with pulmonary fibrosis by Western blot.Results:Compared with the model group,the lung coefficient and HYP de creased in the hormone group (P ＜ 0.05).Lung coefficient and HYP decreased significantly in TCM group (P＜0.01).Compared with the blank group,the expression of TGF-β1 and Smad2 in the lung tissue of the model group increased (P＜0.01) and the expression of Smad7 protein decreased (P＜ 0.01).Compared with the model group,the hormone group and TCM group could significantly reduce the expression of TGF-β1 protein (P＜ 0.01);the hormone group could decrease the expression of Smad2 protein (P ＜ 0.05),and increased the expression of Smad7 protein (P＜ 0.05).TCM group could significantly reduce the expression of Smad2 protein (P＜ 0.01),and TCM group could significantly up-regulated expression of Smad7 protein (P＜ 0.01).Conclusion:Yiqi Huoxue Tongluo decoction can obviously reverse pulmonary fibrosis induced by bleomycin in rats.%目的 观察益气活血通络方对博莱霉素诱导的大鼠肺纤维化影响及作用机制.方法 将60只大鼠按体质量随机分为空白组、模型组、激素组、中药组.除空白组外,其余各组采用博来霉素5 mg/kg行气管注射复制肺纤维化模型.造模后,激素组和中药组给予相应药物灌胃,空白组和模型组以0.9％氯化钠注射液灌胃,每日1次.28 d后处死大鼠,Masson染色观察肺组织病理学,称量肺质量并计算肺系数,Jamall法测定肺组织中羟脯氨酸(HYP)的含量,Western blot法检测大鼠肺组织TGF-β1,Smad2、Smad7蛋白表达.结果 与模型组比较,激素组肺系数、HYP下降(P＜ 0.05),中药组肺系数、HYP显著下降(P＜0.01);与空白组大鼠比较,模型组大鼠的肺组织中TGF-β1和Smad2蛋白表达水平升高(P＜0.01),Smad7蛋白表达水平降低(P＜0.01);与模型组大鼠比较,激素组、中药药能显著下调肺纤维化大鼠的肺组织中TGF-β1蛋白的表达水平(P＜0.01),激素组能够下调肺纤维化大鼠的肺组织中Smad2蛋白的表达水平(P＜0.05)、上调Smad7蛋白的表达水平(P＜0.05),中药组能够显著下调肺纤维化大鼠的肺组织中Smad2蛋白的表达水平(P＜0.01)、上调Smad7蛋白的表达水平(P＜0.01).结论 益气活血通络方可以逆转博莱霉素诱导的大鼠肺组织纤维化情况,调控TGF-β1/Smads信号通路相关因子表达.