Protease treatments on wool have its inherent defect in shrink-proofing because protease will decompose wool through cell membrane complex(CMC). In order to solve this problem, mechanism of enzyme treatments on wool was adequately analyzed and possible enzyme applications were discussed. The mechanism of enzyme treatment on wool was analyzed through weight loss, strength, scanning electron microscopy (SEM), electrophoresis, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and amino acids composition. Based on the results, a possible novel multifunctional enzyme treatment on wool to achieve shrink-resistance was proposed. In order to shorten enzyme treatment time, the rate of proteolysis of wool was investigated. Considering the specificity of proteases, wool composition, and structure of dyes, a better enzyme pretreatment before dyeing to get better dyeability and lower dyeing temperature was discussed.
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