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一种酶解制备低分子量κ-卡拉胶钾的方法及应用

机译:一种酶解制备低分子量κ-卡拉胶钾的方法及应用

摘要

A method for preparing k-potassium carrageenan having a low molecular weight by means of enzymolysis, comprising the following steps: (1) preparing a k-carrageenan aqueous solution having a mass concentration of 0.5-1.5% from k-carrageenan powder in a 40-50°C water bath kettle, stirring uniformly, adding k-carrageenanase, and stirring for enzymolysis for 6-8 h; (2) subjecting an enzymatic hydrolysate to alcohol precipitation to obtain k-carrageenan oligosaccharide having a low molecular weight; and (3) preparing a solution having a mass concentration of 4-10% from the obtained k-carrageenan oligosaccharide, mixing the solution with a potassium chloride aqueous solution having a mass concentration of 0.1-2%, and subjecting the mixed solution to alcohol washing and salt removing to obtain k-potassium carrageenan having a low molecular weight. The method has simple steps and mild conditions; the molecular weight of prepared k-potassium carrageenan having a low molecular weight is stabilized within a small range; and the potassium ion binding rate is high. K-potassium carrageenan can be added into food as a food additive to obtain healthcare products, and can also be prepared into medicines as drugs for preventing or treating hypertension.
机译:一种通过酶解制备低分子量k-角叉菜胶的方法,其包括以下步骤:(1)从40%的k-角叉菜胶粉中制备质量浓度为0.5-1.5%的k-角叉菜胶水溶液。 -50°C水浴锅,搅拌均匀,加入k-角叉菜胶酶,搅拌酶解6-8h; (2)将酶水解物进行醇沉淀,得到低分子量的k-角叉菜聚糖低聚糖。 (3)从得到的κ-角叉菜聚糖低聚糖中制备质量浓度为4-10%的溶液,将其与质量浓度为0.1-2%的氯化钾水溶液混合,并使其混合。洗涤和脱盐以获得具有低分子量的k-角叉菜胶。该方法步骤简单,条件温和。所制得的低分子量K-角叉菜胶的分子量稳定在小范围内。钾离子结合率高。 K-角叉菜胶可以作为食品添加剂添加到食品中以获得保健产品,也可以制成预防或治疗高血压的药物制成药物。

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