黄秋葵水提物抑制α-淀粉酶活性的研究

摘要

目的:研究黄秋葵果荚和种子水提物对α-淀粉酶活力抑制效果.方法:采用3,5- 二硝基水杨酸(DNS)比色法研究不同浓度黄秋葵水提物对α-淀粉酶活力抑制作用、反应时间对抑制效果的影响和抑制类型.结果:黄秋葵果荚水提物对α-淀粉酶活性的抑制率可达84.26％,其半抑制质量浓度(IC50)为24.6mg/mL,且15 min便可以达到最佳抑制效果,抑制类型为可逆抑制.黄秋葵种子水提物也具有较强的α-淀粉酶抑制活性,但抑制效果略低于果荚水提物.结论:黄秋葵水提物中含有α-淀粉酶抑制剂且抑制效果显著,揭示黄秋葵具有抗糖尿病活性和开发治疗糖尿病降血糖药物的价值.%Objective:The aim of this study was conducted to investigate the inhibitory effects of okra peel and seed water extracts against α-amylase.Methods:3,5-dinitrosalicylic acid(DNS)methold was used to study the inhibitory effects of okra water extracts on α-amylase activity,effect of reaction time on inhibitingα-amylase activity and its inhibitory type.Results:the results demonstrated that the inhibitory rate of extraction of okra peel water extracts can reach 80％,the half maximal inhibitory concentration was 24.97mg/mL,and the best inhibitory effect probably appeared at 15 minuter later and Kinetic curve showed that the inhibition type was reversible inhibition.Moreover,okra seed water extracts also exhibited significant α-amylase inhibitory activity,but the inhibitory activity were weaker than peel and seed water extracts.Conclusion:α-amylasee inhibitors existed in the okra water extracts and the inhibition effect was remarkable,giving a clear evidence that okra possessed anti-diabetic activity and could be exploited the diabetes drugs.