HSP70与不稳定性心绞痛患者外周血单核细胞TLR4信号通路活化的相关性研究

摘要

[目的]探讨热休克蛋白70(HSP70)与不稳定性心绞痛(UAP)患者外周血单核细胞TLR4信号通路活化的相关性.[方法]选取2014年12月至2016年3月在本科收治的30例UAP患者及30例稳定性心绞痛(SAP)患者的临床资料,将其分为UAP组及SAP组.同时选择同期本院收治的30例因胸痛疑似急性冠脉综合征(ACS)患者作为对照组,后经冠脉造影显示冠脉正常者.通过ELISA检测各组患者外周血清HSP70水平及单核细胞胞核蛋白NF-κB p65水平,采用流式细胞术检测各组患者外周血单核细胞TLR4表达,并将各组参数两两进行相关性分析.[结果]UAP组患者HSP70水平、单核细胞TLR4阳性表达率与单核细胞胞核蛋白NF-κBp65水平均高于SAP和对照组,差异均具有统计学意义(均P＜0.05);SAP组外周血单核细胞TLR4阳性表达率显著高于对照组,差异具有统计学意义(P＜0.05).UAP组患者外周血清HSP70水平与单核细胞TLR4阳性表达率及胞核蛋白NF-κB p65均具有显著相关性(P＜0.05),其单核细胞TLR4阳性表达率与胞核蛋白NF-κB p65具有显著相关性(P＜0.05);SAP组、对照组,上述指标两两间无显著相关性(P＞0.05).[结论]HSP70可能通过单核细胞TLR4受体参与了UAP患者外周血单核细胞TLR4信号通路的活化.%[Objective]To evaluate relationship between the heat shock protein 70 (HSP70) and the activation of TLR4 signaling pathway in peripheral blood mononuclear cells in unstable angina pectoris (UAP) patients.[Methods]The clinical data of 30 patients with UAP and 30 cases with stable angina pectoris (SAP) who were admitted to our hospital from December 2014 to March 2016 were selected and divided into UAP group and SAP group.At the same time,30 pa tients with suspected acute coronary syndrome (ACS) who were admitted to our hospital in the same period of time were selected as control group who showed a normal coronary artery after coronary angiography.The levels of HSP70 in peripheral blood serum and monocyte NF-κB p65 levels were detected by ELISA.Flow cytometry was used to detect the expression of TLR4 in peripheral blood mononuclear cells of each group,and the correlation of the parameters of each group was analyzed by order.[Results]Levels of HSP70,the positive rate of TLR4 expression in monocytes and the monocyte nuclear protein NF-κB p65 in the UAP group were higher than those in the SAP group and the control group,the difference was statistically significant (mean P ＜0.05);the positive expression rate of TLR4 in peripheral blood mononuclear cells of the SAP group was significantly higher than that of the control group,the difference was statistically significant (P ＜0.05).The HSP70 level in peripheral blood serum of the UAP group was significantly correlated with the positive expression rate of TLR4 and the nuclear protein NF-κB p65 (P ＜0.05);the positive expression rate of TLR4 was significantly correlated with the nuclear protein NF-κB p65 (P ＜0.05);There was no significant correlation on the above indexes between the SAP group and the control group (22) (P ＞0.05).[Conclusion]HSP70 may be involved in the activation of TLR4 signaling pathway in peripheral blood mononuclear cells of UAP patients,through the expression of monocyte TLR4 receptor.