目的 探讨细胞色素CYP2A6基因多态性与COPD易感性的关系.方法 应用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测40例COPD患者及37例健康体检者静脉血细胞色素P450 2A6位点等位基因和基因型.结果 1.COPD患者CYP2A6-163C>A位点基因缺失型(del)和野生型(wt)基因型频率为17.5%、82.5%,健康对照组分别为40.5%、59.5%,两组基因分布频率差异有统计学意义(χ2=5.000,P=0.025),携带CYP2A6wt基因型者较携带CYP2A6del基因型者患COPD风险增加(OR=0.31,95%CI=0.109-0.886,P<0.05).2.在吸烟者中,携带CYP2A6wt基因型者较携带CYP2A6del基因型者患COPD风险增加(OR=0.24,95%CI=0.064-0.920,P<0.05),在不吸烟者中,携带CYP2A6wt、CYP2A6del基因型者之间患COPD风险无明显差异.结论 1.CYP2A6-163C>A基因多态性可能是COPD发病的危险因素.2.野生型(wt)可能为吸烟者患COPD的一个易感因素.%Objective To investigate the association between CYP2A6 polymorphism and susceptibility of chronic obstructive pulmonary disease ( COPD ). Methods 40 COPD patients and 37 healthy people were enrolled in this study, and their venous blood cyto-chrome P450 2A6 allele and genetic type were detected by PCR-RFLP. Results 1. The genotype frequency of gene deletion type ( del ) and wild type ( wt) on CYP2A6-163C > A was 17. 5% and 82. 5% in COPD patients and 40. 5% and 59. 5% in the controls respectively. The difference of gene distribution frequency had statistical significance ( x2 =5.000, P =0.025 ). The COPD patients carrying CYP2A6wt genotype had a higher risk than those carrying CYP2A6del genotype did ( OR=0. 31, 95% 0=0. 109 -0. 886, P <0. 05 ). 2. Smokers carrying CYP2A6wt genotype had a higher risk than those carrying CYP2A6del genotype ( OR = 0. 24, 95% CI - 0. 064 -0.920, P<0. 05 ). There was no significant difference among nonsmokers. Conclusion 1. The polymorphism of CYP2A6-163C > A may be the risk factors causing COPD. 2. Wild type (wt ) may be a predisposing factor of COPD to smokers.
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