目的：探讨碱性成纤维细胞生长因子（bFGF）及转化生长因子β1（TGF-β1）在血管紧张素Ⅱ（AngII）诱导的心脏成纤维细胞（CFs）纤维化不同时期的表达，及其可能作用。方法采用胰酶消化法和差速贴壁法分离1 ~ 3日龄新生SD大鼠的心脏CFs，将培养的第2 ~ 4代CFs随机分为实验组和对照组。对照组培养时不加干预药物；实验组用Ang II（10-6 mol/L）诱导CFs纤维化；培养12、24、48、72 h后，采用ELISA法测上清液胶原含量，采用RT-PCR法检测bFGF及TGF-β1的mRNA表达；Western Blot法检测bFGF及TGF-β1的蛋白表达。结果与对照组相比，实验组CFs的bFGF、TGF-β1 mRNA及蛋白的表达逐渐增高，在不同时间点的差异有统计学意义（P < 0.05），且均与上清液胶原呈正相关（r=0.881 ~ 0.932，P均 < 0.05）；bFGF与TGF-β1 mRNA及蛋白的表达呈显著正相关（r=0.967、0.947，P均 < 0.05）。结论 bFGF与TGF-β1可能协同促进心肌纤维化的发生发展。%Objective To study the expression of basic ifbroblast grouth factor (bFGF) and transforming growth factorβ1 (TGF-β1) in different stages of myocardial ifbrosis (CFs). Methods CFs of neonatal Sprague-Dawley rats were isolated with the method of trypsin digestion and differential anchoring velocity, then cultured in vitro. The generation 2-4 of CFs were used for the experiment and randomly divided into 2 groups:the control group were cultured without AngII , and the test group were cultured with AngII 10-6 mol/L. The test group were cultured for 12, 24, 48, and 72 h respectively, and then the synthesis of col agen were measured by ELISA, the bFGF, TGF-β1-mRNA expression was measured by RT-PCR, and the bFGF and TGF-β1 protein expression was measured by western blot analyses. Results Compared with those of control group, the expressions of bFGF and TGF-β1 both in gene and in protein in the test groups increased gradual y with the timing (P < 0.01). Correlation analysis found that the expression of bFGF mRNA and protein were positively associated with TGF-β1 mRNA and protein (r = 0.967, 0.947, P <0 .05), and both bFGF and TGF-β1 were positively associated with the supernatant col agen. (r = 0.932, 0.881, 0.930, 0.896, P <0 .05). Conclusion bFGF and TGF-β1 may be involved in the occurrence and development of myocardial ifbrosis.
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