急性乙醇中毒对大鼠创伤性脑损伤后GFAP、AQP4表达的影响

摘要

目的：探讨急性乙醇中毒对大鼠创伤性脑损伤（ TBI）后血清及脑组织神经胶质纤维酸性蛋白（GFAP）、水通道蛋白4（AQP4）表达的影响，并分析其分子生物学机制。方法将45只大鼠按体质量随机分为3组，实验1组、2组及对照组，每组15只大鼠。实验1组、2组大鼠分别给予浓度为30％、60％的乙醇（蒸馏水稀释）1 ml／100 g灌胃，对照组大鼠给予相同剂量的蒸馏水灌胃。在灌胃后1 h后对各组大鼠采用改进的Feeney's自由落体硬膜外撞击方法行创伤性脑损伤模型的制作。于大鼠TBI后1 h、6 h、24 h，分别从各组随机抽取5只大鼠抽血，高速离心后测定血清中AQP4、GFAP的含量。各组大鼠抽血后断头处死，迅速完整取出大脑，进行脑组织中AQP4、GFAP的含量测定。结果（1）与对照组比较，实验1组、2组大鼠血清及脑组织中GFAP和AQP4的表达均有显著增加（均P＜0．05）。（2）实验2组比1组大鼠血清及脑组织中GFAP和AQP4的表达增加更明显（均P＜0．05）。（3）随着伤后时间延长，3组大鼠血清及脑组织中GFAP和AQP4的表达越明显。结论急性乙醇中毒可加重大鼠TBI的程度，且血液中乙醇浓度越高，伤后时间越长，TBI的程度越严重。%Objective To investigate the effect of acute ethanol intoxication ( AEI ) on the expression of GFAP and AQP4 in serum and brain tissue after traumatic brain injury ( TBI) in rats. The underlying mechanism was also investigated .Method 45 rats according to body weight were randomly assigned to 3 groups, as experimental group 1,2 and control group, each group has 15 rats.Rats in experiment group 1,2 were given 30%, 60% ethanol separately (1 ml /100g) by stomach irrigation ( ethanol diluted with distilled water ) , the control group was given the same dose of distilled water .Rats in each group underwent improved Feeney 's free falling epidural hitting for traumatic brain injury model 1 h after ethanol administration .After TBI 1 h, 6 h, 24 h, respectively from each experimental group and control group were randomly selected from five rats blood , subsequent high-speed centrifugation , the levels of GFAP and AQP4 in serum was determinated . The rats were killed after blood was drawn and the brain was quickly removed , and the levels of GFAP and AQP4 in brain tissue was determined .Results ( 1 ) Compared with the control group , the expression of AQP4 and GFAP in serum and brain tissues of 1, 2 group were significantly increased (all P<0.05).(2) The expressions of AQP4 and GFAP in serum and brain tissues of the group 2 were significantly higher than those in group 1 ( all P<0 .05 ) .( 3 ) The expression of GFAP and AQP4 in the serum and brain tissue of the three groups was more obvious with the prolonging of the time of injury .Conclusions Acute ethanol intoxication can increase the severity of TBI in rats, and the higher the concentration of ethanol in blood , the longer after the injury,the more after the TBI .