江苏地区汉族人群白细胞介素28B基因多态性与丙型肝炎易感性的关系

摘要

Objective To investigate the association between interleukin-28B (IL-28B)polymorphisms and susceptibility to hepatitis C among the Han population in Jiangsu Province,China.Methods A case-control study was conducted in 88 hepatitis C patients who visi-ted the Second Hospital of Nanjing in 201 1 and 100 healthy controls who underwent physical examination in the same hospital.PCR and di-rect sequencing were used to detect IL -28B single -nucleotide polymorphisms (SNPs) (rs8099917,rs12979860,rs12980275, rs8113007,and rs12972991).Genotypes and alleles were compared between the two groups.Statistical analysis was performed by Pearsonχ2 test;the sample representativeness was evaluated according to the Hardy-Weinberg equilibrium.Results There were no significant differences in sex and age between the two groups (P=0.92 and 0.28).The major genotypes at the five IL-28B SNPs among subjects were TT,CC,AA,AA,and AA.No significant differences were observed between hepatitis C patients and healthy controls in the genotypes and alleles at these IL-28B SNPs (χ2 =0.31,0.50,0.60,0.15,and 0.07,P>0.05 for all;χ2 =0.29,0.48,0.56,0.14,and 0.07,P>0.05 for all).Conclusion IL-28B SNPs (rs8099917,rs12979860,rs12980275,rs8113007,and rs12972991)may not be associated with the susceptibility to hepatitis C among the Han population in Jiangsu Province.%目的：探讨江苏地区汉族人群白细胞介素（IL）28B基因多态性分布及其与丙型肝炎易感性的关系。方法选取2011年南京市第二医院就诊的丙型肝炎患者88名，体检中心健康体检的健康人群100例。应用聚合酶链式反应（PCR）和直接测序法检测丙型肝炎患者和健康人群IL-28B rs8099917、rs12979860、rs12980275、rs8113007和rs12972991位点的单核苷酸多态性（SNP），并统计分析这些位点的基因型和等位基因在两种不同人群中分布的差异。采用Pearson χ2检验进行分析，计算Hardy-Weinberg遗传平衡以判断样本的代表性。结果两者间性别和年龄差异均无统计学意义（P＝0．92、0．28），在检测人群中IL-28B基因5个位点的主要基因型分别是TT、CC、AA、AA、AA；丙型肝炎患者IL-28B基因SNP位点与健康人群在基因型（χ2＝0．31、0．50、0．60、0.15、0.07，P均＞0．05）和等位基因（χ2＝0．29、0．48、0．56、0．14、0．07，P均＞0．05）分布上差异均无统计学意义。结论 IL-28B 的SNPs位点（rs8099917、rs12979860、rs12980275、rs8113007、rs12972991）可能与江苏省汉族人群丙型肝炎易感性无关。