Objective To observe the effect of tetrandrine on the levels of IL - 1 (3, IL - 6 and TNF - a in serum and articular cavity fluid in collagen induced arthritis ( CIA ) rats, in order to investigate the possible therapeutic effects of tetrandrine on CIA. Methods Male Wistar rats were used to make CIA model. The CIA rats were randomly divided into normal groups ( 10 rats ), tetrandrine group ( 10 rats ) and CIA control group ( 10 rats ). The rats in tetrandrine group were administered with tetrandrine at 80mg/kg/day intramuscularly. CIA control group and normal rats were given normal saline instead. The rats were sacrificed on day 30 after tetrandrine administration. IL -1 (3, IL - 6 and TNF - a in serum and articular cavity fluid were detected by enzyme linked immunosorbent assay ( ELISA ). Results Compared with normal group, the levels of IL -1(3, IL-6 and TNF - a in serum and articular cavity fluid in CIA control group were increased ( P <0.0l). The levels of IL - 1 (3, IL-6and TNF - a decreased significantly in tetrandrine group ( Tetrandrine v. s. CIA control group P <0. 01 ). Conclusion This study demonstrates that tetrandrine can modulate the production of IL - 1 (3, IL-6 and TNF - a, which maybe one of the mechanisms that tetrandrine treats CIA and rheumatoid arthritis.%目的 观察汉防己甲素对胶原诱导的关节炎(CIA)大鼠关节腔和外周血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响,探讨汉防己甲素治疗CIA的相关机制.方法 以胶原诱导的关节炎Wistar大鼠为动物模型,将大鼠分成正常组、模型组和汉防己甲素组.正常组、模型组注射0.9%氯化钠溶液,汉防己甲素组按 80 mg /kg后腿肌肉注射,1次/d,30 d处死动物,采用酶联免疫吸附法( ELISA)检测大鼠血清和膝关节腔液中IL-1β、IL-6、TNF-α含量.结果 与正常对照组相比,CIA模型组大鼠关节腔液和血清中 IL-1β、IL-6、TNF-α含量升高(P< 0.01);经过汉防己甲素治疗后,关节腔液和血清中IL-1β、IL-6、TNF-α含量降低,与模型组相比,差异有统计学意义(P<0.01),而与正常组比较,差异无显著性.结论 汉防己甲素可以通过调节细胞因子变化而达到治疗CIA,这也可能是汉防己甲素治疗类风湿关节炎的机制之一.
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