溃疡性结肠炎患者肠道菌群与血TNF-α、内毒素水平的关系

摘要

Objective To explore the correlation of characteristics of intestinal flora with serum levels of TN - a and endotoxin in patients with ulcerative colitis. Methods Fifty - seven patients diagnosed as ulcerative colitis including 28 patients at remission stage ( remission group ) and 29 patients at active stage ( active group ). Ten healthy persons were selected as control group. Selective culture mediums were used for counting the amount of bacteria. The serum levels of TJNF - α and endotoxin were determined in every patient of 2 groups. Results The amount of Lac-tobacillii and Bifidobacteria in patients of active group was significantly lower than that of patients in remission group and control group ( all P <0. 01 ). The serum levels of TJNF - α and endotoxin in patients of active group were significantly higher than those of patients in remission group and control group ( P < 0. 01 ). Negative correlation was found between the amount of Lactobacillii and serum levels of TJNF - α and endotoxin ( r -- 0. 860 , - 1. 038 : P < 0. 01 ) , and negative correlation was observed between the amount of Bifidobacteria and serum levels of TJNF - α and endotoxin ( r - - 0. 932 , - 0. 843 : P < 0. 01 ). Conclusion The disorder in intestinal flora can be found in patients with ulcerative colitis. It is beneficial for determining serum levels of TJNF - αand endotoxin to evaluate the severity of disease in patients with ulcerative colitis. There is significant correlation between intestinal flora and serum levels of TJNF - α and endotoxin in patients with ulcerative colitis.%目的 探讨溃疡性结肠炎(UC)患者肠道菌群特点及血中肿瘤坏死因子(TNF)-α、内毒素含量变化,为UC治疗和病情评估提供依据.方法 选取确诊的57例UC患者,其中缓解期28例,活动期29例,10例健康体检者作对照.运用可培养细菌菌群分析方法,对每例受试者粪便进行分析;清晨空腹抽血酶联免疫吸附(ELISA) 法测TNF-α含量,采用鲎试剂三肽显色基质偶氮法测内毒素水平.结果 UC活动期组与缓解期组、对照组比较乳酸杆菌数量明显减少,差异显著(P<0.01);双岐菌UC缓解期组数量少于对照组(P<0.05),活动期组数量明显减少,与缓解期组、对照组比较均具有显著性差异(P<0.01);UC活动期组内毒素水平明显升高,与缓解期组、对照组比较均具有显著性差异(P<0.01);UC活动期组TNF-α水平明显升高,与缓解期组、对照组比较均具有显著性差异(P<0.01);乳酸杆菌数量与血桨内毒素水平、血清TNF-α含量均呈负相关(r=-0.860、-1.038,P<0.01);双岐菌数量与血桨内毒素水平、血清TNF-α含量均呈负相关(r=-0.932、-0.843,P<0.01).结论 UC患者存在菌群失调;测定UC患者血TNF-α、内毒素水平有益于评估病情.