Objective In order to explore the relatively protective effect of losartan, carvedilol and ginseng on apoptosis in rats with ischemia/reperfusion damage, and the incremental number of myocardial apoptosis in rats with ischemia/reperfusion was compared after treatment with losartan, carvedilol and ginseng. Methods The ischemia/reperfusion model of adult Wistar rats were established and divided into operation control group, losartan treatment group, carvedilol treatment group, ginseng treatment group and sham operation group. All rats were fed with drugs or normal saline, and they were killed in forty - eighth hours after operation. The number of apoptotic cells were detected and counted in myocardium at the same ischemia/reperfusion region. Results The amount of apoptotic myocardium in operation control group, losartan treatment group, carvedilol treatment group, ginseng treatment group and sham operation group were 37.53±8.75, 8.75 ±4.21, 7.63 ±3.97, 6.50±3.56 and 0. 18 ±0.91 respectively in every visual field at higher magnification. The numbers of apoptotic cells in sham operation group were significantly lower than those of ischemia/reperfusion group of operation control group, losartan treatment group, carvedilol treatment group and ginseng treatment group( P 0.05). There was also no significant difference between carvedilol treatment group and ginseng treatment group( P > 0.05). But the numbers of apoptosis in losartan treatment group were significantly higher than those of ginseng treatment group ( P < 0.05). Conclusion Losartan, carvedilol and ginseng may depress the increase of myocardial apoptosis caused by ischemia/reperfusion. The depressed function of carvedilol and ginseng may be more stronger than that of losartan. The effect of depression in apoptosis of carvedilol and ginseng may bernsame.%目的 通过比较氯沙坦、卡维地洛和人参对大鼠心肌缺血再灌注后细胞凋亡数目的影响,了解氯沙坦、卡维地洛和人参对大鼠心肌缺血再灌注损伤保护作用的相对强度.方法 制作成年Wistar大鼠心肌缺血再灌注模型,分为手术对照组、氯沙坦治疗组、卡维地洛治疗组、人参治疗组和假手术组共5组,分别予药物或生理盐水,于术后48 h处死取相同缺血再灌注区部位心肌检测计数凋亡细胞数.结果 手术对照组、氯沙坦治疗组、卡维地洛治疗组、人参治疗组和假手术组心肌细胞凋亡数分别为37.53±8.75个/高倍视野、8.75±4.21个/高倍视野、7.63±3.97个/高倍视野、6.50 ±3.56个/高倍视野和0.18±0.91个/高倍视野,手术对照组、氯沙坦治疗组、卡维地洛治疗组、人参治疗组等缺血再灌注后心肌细胞凋亡数均显著高于假手术组(P ＜0.01),手术对照组显著高于氯沙坦治疗组、卡维地洛治疗组和人参治疗组(P＜0.01),氯沙坦治疗组与卡维地洛治疗组心肌细胞凋亡数目无显著差异(P＞0.05),卡维地洛治疗组与人参治疗组也无显著差异(P＞0.05),但氯沙坦治疗组显著高于人参治疗组(P＜0.05).结论 氯沙坦、卡维地洛和人参均可抑制缺血再灌注后的心肌细胞凋亡增加,人参和卡维地洛抑制缺血再灌注后心肌细胞凋亡作用可能优于氯沙坦,而人参和卡维地洛抑制缺血再灌注后心肌细胞凋亡作用可能基本相同.
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