不同剂量红景天对阿霉素致心肌损伤大鼠cTnI、BNP、AI的影响及剂量-疗效关系模型的建立

摘要

Objective To obseve the cardiac troponin I(cTnI),brain natriuretic peptide(BNP)and cardiomyocyte apoptosis(AI)in rats with Doxorubicin induced cardiomyocyte injure and the effect of different dosage of Rhodiola on this pathological process. In order to find out if Rhodiola plays a positive role in preventing chemotherapy induced cardiomyocyte injure. Methods 60 model rats with Doxorubicin induced car-diomyocyte injure were divided into six groups randomly(model group,2 g Rhodiola group,3 g Rhodiola group,4 g Rhodiola group,5 g Rhodiola group and 6 gram Rhodiola group). Each group was given intraperitoneal injection of Doxorubicin with 2 milligram per kilogram once a week for six weeks,meanwhile the experimental groups were given intragastric administration of Rhodiola decoction(2 ml each time,the dosage is 2 g,3 g, 4 g,5 g and 6 g raw herba Rhodiola per kilogram separately)every day during the experiment. The amount of hair,body weight,ardiac troponin Ⅰ(cTnI),brain natriuretic peptide(BNP)and cardiomyocyte apoptosis(AI)before and after the experiment were compared. Results The a-mount of hair and body weight of rats in the six groups were almost the same. The differences of cardiac troponin Ⅰ(cTnI),brain natriuretic pep-tide(BNP)and cardiomyocyte apoptosis(AI)decreased from 2 g Rhodiola group,3 g Rhodiola group to 4 gram Rhodiola group(P <0.05), while in 4 g Rhodiola group,5 g Rhodiola group and 6 g Rhodiola group,the differences were not significant. Differences in all experimental groups were smaller than those in control group. No death happened during the experiment. Conclusion Rhodiola is effective in diminishing the adverse effects of adriamycin on heart and is trivial toxicity with well tolerance.%目的 观察阿霉素致心肌损伤大鼠的血清肌钙蛋白I(cTnI)、脑钠肽(BNP)、心肌细胞凋亡指数(AI)的变化及不同剂量红景天对该变化的影响,从而了解红景天对化学药物导致的心肌损伤的保护作用.方法 利用阿霉素致心肌损伤大鼠模型60例,随机分成模型组、红景天干预组(5个不同剂量红景天干预)6组.模型组单纯阿霉素组每周1次腹腔内注射阿霉素2 mg/kg,共6周;红景天干预组大鼠每周1次腹腔内注射阿霉素2 mg/kg,共6周.同时每天联合给予红景天煎剂灌胃(2 ml,红景天剂量分别为2 g、3 g、4 g、5 g、6 g生药/kg体重).观察用药前后大鼠毛发、体重、cTnI、BNP、AI情况的变化.结果 观察6组参与治疗的大鼠,不同红景天剂量组体重和毛发变化基本一致.各组用药前后BNP、cTnI、AI差值比较:随着红景天剂量提高,红景天2 g、3 g、4 g组中上述三个观察指标差值逐步减小(P <0.05);但剂量加至4g、5g、6g时,上述三个指标未见显著差异;所有组中,红景天干预组均显著低于模型组.所有大鼠均未见死亡.结论 红景天能有效减轻阿霉素所致大鼠的心脏毒性,有效改善cTnI、BNP、AI的升高,并且在一定范围内,随剂量增加其效果亦增加,且口服红景天毒性较低,耐受性好.