Objective To explore the associations of single nucleotide polymorphisms (- 597 G > A、- 572 C > G、- 174 G > C)of IL -6 and single nucleotide polymorphisms (- 238 G > A、- 308 G > A)of TNF-α gene promoter with breast cancer susceptibility. Methods DNA Sequencing technique was used to detect the gene polymorphism of IL-6 (- 597 G > A、- 572 > G、- 174 G > C)and TNF-α (- 238 G > A、- 308 G > A)in 136 cancer women with breast cancer and 150 healthy women. Association analysis based on unconditional logistic regression was carried out to determine the odds ratio (OR)and 95％ confidence interval (95％ CI)for the SNPs between different genotype and breast cancer. Results The frequency of IL-6-572 C > G polymorphism genotypes was not significantly different between cases groups and the controls. Compared with the IL-6-572 CC genotype,the CG genotype increased the risk of breast cancer (OR = 1. 841,95％ CI:1. 115 ～ 3. 040,P= 0. 017). Conclusion The-572 G > C polymorphism of IL-6 gene promoter was associated with breast cancer. The CG genotype increased the risk of breast cancer.%目的 探讨白细胞介素6(IL-6)基因rs1800797(-597 G>A)、rs1800796(-572 C>G)、rs1800795(-174 G>C)及肿瘤坏死因子 α(TNF-α)基因的启动区rs1800629(-238G>A)、rs361525(-308 G>A)单核苷酸多态性(SNP)与乳腺癌易感的相关性.方法 应用DNA测序方法检测136例乳腺癌患者及150例正常女性IL-6基因启动区-597 G>A、-572 G>C、-174 G>C及TNF-α基因-238G>A、-308 G>A位点的基因多态性.应用 χ2检验法分析乳腺癌组和正常对照组之间基因型分布的差异,并采用非条件Logistic回归分析计算风险(OR)比及95％可信区间(95％CI),评估各基因型与乳腺癌发病风险的相关性.结果 IL-6基因启动子-572 C>G位点基因型分布在乳腺癌组和正常组之间存在显著差异(PC基因多态性与乳腺癌易感性相关,携带CG基因型的女性患乳腺癌的风险增加.
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