DNA甲基转移酶抑制剂改变小孢拟盘多毛孢次级代谢产物的研究

摘要

DNA甲基转移酶抑制剂5氮杂胞苷导致了植物内生真菌小孢拟盘多毛孢次级代谢产物的明显变化.分析发酵液萃取物分离到一个新化合物4'-formamidophenyl-5-methoxybenzoate (1),和七个已知的聚酮4-hydroxybenzoic acid (2),LL-P880α (3),1'-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one (4),pestalotiollide B (5),pestalotiopyrone G (6),endocroein (7),and 2'-hydroxy-6'-hydroxymethyl-4'-methylphenyl-2,6-dihydroxy-3-(2-isopentenyl)benzoate (8).液相分析结果显示除了4以外,其他所有化合物都是新诱导产生的.所有化合物被证明没有明显的抗真菌活性.%5-Azacytidine,a DNA methyltransferase inhibitor,led to significant changes in the secondary metabolism of the plant endophytic fungus,Pestalotiopsis microspora.Analysis of the culture broth extract led to the isolation of a new compound,4'-formamidophenyl-5-methoxybenzoate (1),along with seven known polyketides,4-hydroxybenzoic acid (2),LL-P880α (3),l'-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one (4),pestalotiollide B (5),pestalotiopyrone G (6),endocrocin (7) and 2'-hydroxy6'-hydroxymethyl-4'-methylphenyl-2,6-dihydroxy-3-(2-isopentenyl)benzoate (8).HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites.In addition,all compounds were proved to be devoid of significant antifungal activity in the bioassays.