北五味子的超临界萃取及产物的HPLC-APCI-MS/MS分析

摘要

优化超临界流体萃取北五味子中木脂素类成分的提取工艺,对萃取物中所含有效成分的归属进行分析鉴定.以五味子总木脂素为评价指标,采用正交实验法和不同影响因素系统考察法对CO2 -SFE萃取北五味子中木脂素类成分提取工艺进行优化,利用液相色谱-大气压化学电离质谱联用法(HPLC-APCI-MS/MS)分析鉴定产物结构.确定了最佳的超临界提取工艺,即压力为25 MPa,温度为45℃,提携剂浓度为90％乙醇,并对萃取产物中所含的10种木脂素类化合物进行归属鉴别.证明优化的工艺与其他常规提取工艺相比较科学合理,具有较强的稳定性和有一定的实用价值.%The orthogonal test was used to optimize CO2-SFE process of the lignans in Schisandra chinensis fruits with content of total lignan as index, and the extraction prod-ucts were analyzed by HPLC-APCI-MS/MS. The optimal extraction procedure is as fol-lows: the extraction pressure is 25 Mpa, the extraction temperature is 45 °C ,and the con-centration alcohol is 90%. Ten structures of extraction products are confirmed by HPLC-APCI-MS/MS. The optimum extraction procedure is reasonable and stable. Compared SFE with traditional methods, the optimum extraction procedure is excellent and valuable.