2,4-二硝基氟苯柱前衍生RT-HPLC法测定γ-氨基丁酸

摘要

以富含γ-氨基丁酸(GABA)的大豆胚芽乳、黄酒、有机糙米、绿茶和杨梅为研究对象,采用2,4一二硝基氟苯(FDNB)柱前衍生和等度洗脱,建立一种快速测定γ-氨基丁酸含量的RT-HPLC方法.该方法简便,分析时间短(<7 min),灵敏度高,稳定性和重现性好.优化后色谱条件如下:Hypersil ODS2 C18,流动相为乙腈:水:磷酸盐缓冲液(pH=7.2,体积比为20:20:60),检测波长为360 nm.在最适条件下,γ-氨基丁酸的线性检测范围为0.016～1.000 mg/mL,线性关系良好,回收率为96.5%～104.0%.%In this paper,a method for determining γ- aminobutyric acid in Chinese bayberry was developed. It was based on a precolumn derivatization of GABA with 2,4 -dinitrofluorobenzene (FDNB) and isocratic elution by RT - HPLC. It was simple, rapid (less than 7 minutes), steady, sensitive and accurate. The optimized chromatographic conditions were as follows: Hypersil ODS2 C18, eluent consisted of 20％ acetonitrile,20％ water and 60％ phosphate buffer solution. GABA was determined with UV detector at 360nm,and good linearities were observed within a range from 0. 016 to 1.0 mg/mL. The recoveries were 96.5％ ～ 104.0％ for GABA.