化学发光法对花生多肽的体外抗氧化活性和对DNA损伤的保护作用的研究

摘要

采用多个化学发光体系系统地研究了花生多肽的体外抗氧化活性及其对DNA损伤的保护作用.运用邻苯三酚-鲁米诺化学发光体系测定了花生多肽对超氧阴离子的清除作用,硫酸铜-邻菲哕啉-抗坏血酸-双氧水、硫酸亚铁-鲁米诺-双氧水和硫酸亚铁-鲁米诺3个体系测定了花生多肽对羟基自由基的清除作用,双氧水-鲁米诺体系测定了花生多肽对体外双氧水的清除作用,采用硫酸铜-邻菲哆啉-抗坏血酸-双氧水-脱氧核糖核酸测定了花生多肽对体外DNA损伤的保护作用.试验结果表明花生多肽具有较好的体外清除活性氧和保护DNA损伤的活性.花生多肽可作为开发抗氧化产品的新资源.%The antioxidant activities of peanut protein peptides(PPs) were investigated by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the pyrogallol- luminol system, the CuSO4 -Phen -VC -H2O2 system, and the luminol -H2O2 system,respectively. DNA damage preventing effect of PPs was determined by the CuSO4 -Phen- VC -H2O2 - DNA CL system. The results showed that PPs had good scavenging potency on ROS in different systems. PPs can be used as new resources of the antioxidation production.