本研究建立了一种利用碳纳米管(Carbon Nanotubes,CNTs)富集茶油中多酚新型前处理方法.在考察CNTs物化性质、吸附介质、解吸溶液等对多酚吸附、解吸规律的基础上,采用经纯化的管径在20 ～ 30nm、携带-COOH的CNTs为吸附剂,1∶10的料液比,以100 r/min、10℃恒温振荡2h为吸附条件,以丙酮为解吸液进行超声辅助解吸,结果发现:采用CNTs富集茶油中的多酚,用于补偿不完全吸附和解吸的补偿因子为2.032.当茶油中的多酚质量浓度在4.4～93.0 μg/g,富集后的解吸液的吸光度与茶油多酚浓度呈线性关系,相关系数(r2)为0.935 1,方法最低检出限为3.24 μg/g,加标回收率为87.99％ ～ 110.95％,相对标准偏差(RSD,n=6)为8.38％ ～ 10.74％,校正后的检测结果与固相萃取法的结果无显著性差异(P＞0.05).研究结果表明采用CNTs为吸附剂富集、分析茶油中的多酚成分具有稳定性好、准确度高等优点,为茶油多酚的检测提供了一条新途径.%This study developed a new pre-treatment method of polyphenol in oil-tea camellia seed oil through the accumulation of CNTs.On the basis of investigating the physico-chemical property of CNTs,adsorption medium,desorption solution on the adsorption and desorption of polyphenol standard product,we took purified CNTs as adsorbent,which were 20 ～30 nm in diameter and carried much-COOH,1∶10 as material/liquid ratio.and the adsorption conditions were as follows:shaking table at constant temperature of 10 ℃ by 2 h,the velocity was 100r/min.After that CNTs were desorpted by 20 mL actone added with ultrasonic,which repeated 3 times.Finally we found that the compensate factor of the method which was used to compensate for incomplete adsorption and desorption was 2.032.When the concentration of polyphenol in oil-tea camellia seed oil was between 4.4 and 93.0 μg/g,the absorbance of desorption solution presented a good linear relationship with the concentration of polyphenol in oil-tea camellia seed oil,the correlation coefficient (r2) was 0.935 1.The minimum determination limit of the method was 3.24 μg/g and the recoveries were between 87.99％ and 110.95％ with relative standard deviation (RSD,n =6) between 8.38％ and 10.74％.There's no significant difference between the results of this method and those of Dio-SPE (P ＞ 0.05).The results showed that the method which taken CNTs as adsorbent to analysis the polyphenol in oil-tea camellia seed oil had the advantage of high stability and accuracy,which provided a new mode of polyphenol determination in oil-tea camellia seed oil.
展开▼
