目的 探讨基质金属蛋白酶(MMP)蛋白在百草枯(PQ)诱导的大鼠肺组织中的表达.方法 雄性SD大鼠40只,随机分为2组,每组20只.盐水对照组(NS组):一次性腹腔注射生理盐水8.5 mI/kg,模型组(PQ组):一次性腹腔注射百草枯15.0 mg/kg,分别于给药后5d、28 d处死动物,HE染色观察肺组织炎症、纤维化程度,免疫组化法观察各组大鼠MMP3、MMP8蛋白表达情况.结果 建模5dPQ组大鼠肺组织炎症程度高于NS组(P＜0.05),建模28dPQ组大鼠肺组织炎症、纤维化程度高于NS组(P＜0.05),模型组在腹腔注射后5d时肺泡间隔和肺泡腔内炎性细胞浸润,成纤维细胞增多,28 d时成纤维细胞大量增生,但外周肺实质炎症减轻.MMP3、MMP8在NS组大鼠肺脏中即有表达,但表达较弱,模型组大鼠肺组织中MMP3、MMP8的表达于5d时表达增强,此后28 d时已基本接近正常水平.结论 百草枯染毒后大鼠肺组织早期以炎性反应为主,成纤维细胞增多,晚期以纤维化为主,MMP3、MMP8蛋白在百草枯致肺纤维化大鼠肺组织炎性反应期中表达增强,提示它们可能是PQ致肺纤维化的标志物.%Objective To investigate the expression of MMP3 and MMP8 in paraquat-induced pulmonary fibrosis in rats. Methods Forty SD rats (random sex) were randomly divided into negative control group (NS group,n =20) and pulmonary fibrosis model group (PQ group,n =20). Rats in pulmonary fibrosis model group were intraperitoneal injected with PQ of 15.0 mg/kg. Rats of other group were in-traperitoneal injected with the same dose of saline. Animals in the two groups were sacrificed on day 5 and day 28. The lung were stained by HE. The expressions of MMP3 and MMP8 in lungs were observed by immunohistochemistry. Results The pulmonary inflammation in pulmonary fibrosis model group was significantly serious than that of control group, pulmonary fibrosis in PQ was also significantly serious than that of control group. Expression cystourethrography:a small quantity of MMP3 and MMP8 were measured in normal lung,and the expression increased significantly after administration of paraquat (P < 0.05). Different expressions of MMP3 and MMP8 in different days after paraquat administration were observed. Conclusion The expressions of MMP3 and MMP8 in different days after paraquat administration were different. The expressions of MMP3 and MMP8 were significantly serious in the lung of pulmonary fibrosis model group.
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