重度子痫前期患者血清对外周血NK细胞杀伤活性的影响

摘要

Objective To study the effect of the sera ~om severe preeclamptic patients on killing ability of peripheral blood NK cells.Methods NK cells from healthy non-pregnant women were incubated with 10％ ,20％ and 40％ serum from severe preeclampsia patients and normal pregnant women for 20 hours.The effect of the sera on NK killing ability to K562 cells was analyzed by MTT method.Results In 20％ severe preeclamptic sera group,NK killing ability to K562 cells [(48.68±8.47)％]was significantly increased compared with that in normal pregnant group [(35.21±8.24)％](P ＜ 0.01 ).The killing ability of NK cells was (39.98±7.65)％ in 10％ severe preeclamptic sera group,while (58.33±7.45)％ in 40％ severe preeclamptic sera group.There were significant differences among 10％,20％and 40％ severe preeclamptic sera group (F =24.326, P ＜ 0.01 ).Conclusion The sera from severe preeclamptic patients might increase the killing ability of NK cells.%目的 研究重度子痫前期患者血清对NK细胞杀伤活性影响.方法 重度子痫前期患者组(病例组)、正常妊娠妇女组(对照组)各15例,采用1:1配比的病历对照研究.采用Percoll不连续密度梯度方法分离健康非孕妇女NK细胞,用10%、20%、40%浓度重度子痫前期患者血清及20%正常妊娠妇女血清孵育NK细胞20 h,.采用MTT法检测不同血清对NK细胞杀伤活性影响.结果 在用20%重度子痫前期患者血清及正常妊娠血清孵育20 h后,重度子痫前期组NK细胞杀伤活性为(48.68±8.47)%,正常妊娠组为(35.21±8.24)%.与正常妊娠组相比,重度子痫前期组NK细胞杀伤活性增强,差异有统计学意义(P＜0.01).在重度子痫前期组,用10%浓度血清孵育20 h后,NK细胞杀伤活性为(39.98±7.65)%,40%浓度血清组为(58.33±7.45)%.重度子痫前期组中10%、20%、40%浓度血清组间两两相比,差异有统计学意义(F＝24.326,P＜0.01).结论 子痫前期患者血清中某些因子可使NK细胞杀伤活性增强.