白念珠菌支气管肺感染大鼠肺组织Toll样受体2和IL-1O的表达及意义

摘要

Objective Basing on the pathology of rats with Candida albicans infection through bronchus,we investigate the TLR2 expression and the secretion of IL-10 in lungs of rats,as well as the relationship between them. Methods Rats in immunodeficiency and infection group were injected with 80 mg/kg Hydrocortisone every two days for seven times. Rats in Infected group were injected with 0.2 mL suspension of Candida albicans. Pathological staining was used to detect the severity of the infection. Immunohistochemistry and EL1SA were used to detect the expression of TLR2 and IL-10. Results Level of TLR2 and IL-10 in the infected rats are higher than the control rats,and there' s no correlation between the two indexes. Conclusion The infection of Candida albicans could increase the expression of TLR2 and IL-10 in lung,which may affect the infection.%目的 建立大鼠白念珠菌支气管肺感染模型,观察感染后肺Toll样受体2(TLR2)和IL-10水平的变化,探讨TLR2和IL-10在念珠菌支气管肺感染中的作用及意义.方法 建立白念珠菌支气管肺感染大鼠模型,观察感染后肺病理学改变,用免疫组化法检测感染后3d、7d肺组织TLR2的表达,应用ELISA法测定肺组织匀浆上清液中IL-10的水平.结果 感染后肺组织TLR2和IL-10的水平较对照组比较显著升高(P＜0.05),且IL-10随着感染的进程,有升高趋势(P＜0.05)；但两指标之间无显著统计学相关性(P＞0.05).结论 白念珠菌支气管肺感染后肺组织TLR2和IL-10水平升高,可能参与白念珠菌感染的发生和炎症的加重.