米氮平对抑郁症大鼠海马凋亡相关蛋白表达的调节作用

摘要

目的 研究米氮平对抑郁症大鼠模型学习记忆、海马凋亡蛋白Caspase-3和凋亡抑制蛋白Bcl-2异常表达的调节作用.方法 将20只大鼠随机分为对照组、抑郁组和治疗组,制备抑郁症模型及米氮平治疗模型,采用Morris水迷宫实验方法评价学习记忆,应用蛋白免疫印迹杂交方法分析海马Caspase-3蛋白和Bcl-2蛋白表达变化.结果 与对照组比较,抑郁组大鼠在水迷宫实验目标象限内游动时间显著减少(P＜0.01),海马Caspase-3蛋白表达水平显著增高(P＜0.05),Bcl-2蛋白表达水平显著降低(P＜0.05).治疗组大鼠在水迷宫实验目标象限内游动时间较抑郁组增加,海马Caspase-3蛋白表达水平较抑郁组降低,Bcl-2蛋白表达水平较抑郁组升高(P均＜0.05).结论 抑郁症大鼠学习记忆力下降、海马Caspase-3蛋白异常高表达、海马Bcl-2蛋白异常低表达,米氮平使抑郁症大鼠学习记忆力、海马Caspase-3蛋白表达、海马Bcl-2蛋白表达逆转.%Objective To investigate the effects of mirtazapine on learning and memory and on the expression of hippocampal Caspase-3 and Bel-2 protein in rat depression model. Methods Rats were divided into control,depression and treatment group. Depression model and mirtazapine treatment model were prepared,followed by Morris water maze test to evaluate learning and memory,and western blot to assess hippocampal Caspase-3 and Bcl-2 protein expression. Results Rats in the depression group showed significantly reduced swim time spent in the targeted quadrant of the morris water maze,elevated Caspase-3 protein levels,and decreased Bcl-2 protein levels. Conclusion Depression model rats showed impaired learning and memory .abnormal expression of hippocampal Caspase-3 and Bcl-2 protein,while mirtazapine attenuated the abnormalities.