基于线粒体12S rRNA基因序列探讨湖鲚、短颌鲚物种有效性

摘要

The sequences of mitochondrial 12S rRNA genes for Coilia nasus, Coilia nasus taibuensis and Coilia brachynathus were determined using PCR sequencing technology. Based on thel2S rRNA sequences, the phylogenetic trees were reconstructed by maximum parsimony (MP), maximum-likelihood (ML) and Bayesian (BI), using Engraulis japonicus and Coilia lindmani as outgroups. As a result, The trees of concatenated dataset showed that Coilia brachygnathus and Coilia nasus taibuensis didn~ t form reciprocal monophyly. Therefore, Coilia brachygnathus and Coilia nasus taibuensis, as geographical populations of Coilia nasus were supported in this study.%用PCR技术扩增并测序获得了刀鲚、湖鲚和短颌鲚的线粒体12SrRNA基因序列。结合GenBank上已公布的序列数据，以日本鳗、林氏鲚为外群，采用最大简约法（MP）、最大似然值法（ML）和贝叶斯分析（BI）法构建了系统发育树。结果显示短颌鲚、湖鲚均末形成独立的进化支，因此，支持短颌鲚和湖鲚为刀鲚的淡水生态型种群，而并非有效物种的观点。