不同冷冻方法对小鼠卵母细胞冻存的影响

摘要

Objective:To explore the effects of different freezing methods on mouse oocyte survival rate and growth potential. Methods:Using vitrification technology,choose open carrier with enclosed carrier were cryopreserved with mouse cumulus-oocyte complex with cumulus cells (COC)or without cumulus cells (OD),after recovery,for in vitro fertilization and embryo culture.Results:The open carrier cryopreserved live egg number group 76.79%,slightly higher than the 72.98% enclosed carrier cryopreserved had no statistical significance(P >0.05);In two group of the COC group was significantly higher than that of DO group,the survival rate of eggs,statistically significant(P <0.05).The COC group of fertilization rate,blastocyst rate slightly higher than the DO,no statistical difference (P >0.05).Conclusion:(1)the enclosed carrier effectively avoids the potential pollution problems,but different carrier on eggs survival rate had no significant effect.(2)cumulus-cells in the vitrification freezing oocytes play a protective role,and for late-stage embryonic development has certain improvement.%目的：：探讨不同冷冻方法对小鼠卵母细胞存活率及其发育潜能的影响。方法：采用玻璃化冷冻技术,选取开放式载体与封闭式载体分别冻存带卵丘细胞(COC)或剥除卵丘细胞(OD)的小鼠卵丘复合体,复苏后分别作体外受精,胚胎培养。结果：开放式载体冻存组存活卵数76.79%略高于封闭式载体冻存组72.98%,无统计学意义(P >0.05)；两组内 COC 组的卵子存活率显著高于 DO 组,但有统计学差异(P <0.05),COC 组的受精率、囊胚率略高于 DO 组,无统计学差异(P >0.05)。结论：(1)封闭式载体有效避免了潜在的污染问题,但不同载体对卵子存活率无显著影响。(2)卵丘细胞在玻璃化冷冻卵母细胞中可以起到保护作用,并对胚胎后期的发育有一定的改善。