DNA混合分析技术的单体型频率估计方法

摘要

DNA混合分析技术的广泛使用,能够明显降低研究的工作量和成本,然而仍然有其自身局限,例如损失了大量个体遗传信息、测量误差较大等.为了尽量克服DNA混合分析技术的自身问题,其检测和分析方法在不断发展与完善.在单体型频率估计方面,基于最大期望(expectation-maximization,EM)算法的新方法不断涌现,如HaploPool算法和PoooL算法,其准确性、实用性均增强.%DNA pooling, a fast and economic study strategy, is widely used in areas of scientific research.In spite of various limits, researchers are making their efforts to improve DNA pooling toward a more perfect direction, including allele frequency detection and estimation of haplotypes.In haplotype estimation, more and more analyzing methods originated from the expectation-maximization algorithm have appeared, with improved accuracy and practicality, such as HaploPool algorithm and PoooL algorithm.