LIN28B 在结肠癌中的表达及其临床意义

摘要

目的 探讨结肠癌组织中LIN28B的表达及其临床意义.方法 选择美国Biomax的结肠癌及正常组织组合微阵列芯片及2003年1月至2004年12月南方医科大学南方医院的149例手术切除结肠癌石蜡标本作为研究对象.用免疫组化法检测组织芯片和石蜡切片中LIN28B蛋白表达并评分.采用χ2检验比较不同LIN28B表达水平和结肠癌病理性特点包括组织分级、恶性肿瘤(tumor,node,metastasis,TNM)分期及淋巴/远处转移之间的关系.结果 LIN28B在结肠癌组织中相比于正常结肠组织高表达,其高表达与肿瘤的低分化、TNM Ⅲ-Ⅳ期、结肠癌局部淋巴结转移及远处转移有关.多因素Logistic回归分析显示TNM分期是LIN28B高表达的独立危险因素.结论 LIN28B表达与结肠癌病理特征密切相关,有望作为判断结肠癌患者预后的指标.%Objective To invesligale and evaluale the clinical significance of LIN28B expression in coton cancer.Methods Immunohistochemislry was performed to deled the LIN28B expression in coton cancer lissue microarrays and surgically resecled paraffin-embedded lissues. The expression of LIN28B was scored by 2 palhotogisls, and was analyzed using x2 Lest to exptore ils influences on the palhotogic characlerislics of coton carcinoma. Results LIN28B was upregulaled in coton cancer Lissue compared to normal mucosa, and iLs overexpression correlaLed with poor differenLiaLion, higher TNM grade ( III -IV ) , lymph node and disLanL meLasLasis of coton carcinoma. Conclusion LIN28B overexpression correlaLes with aggressive paLhotogic characLeristics of coton carcinoma, and iL may serve as a prognosLic factor of coton cancer paLients.