帕金森大鼠消化道组织中蛋白质NHERF家族和CAL的表达变化

摘要

Objective To study the expression of NHERF family protein and CAL protein in the digestive system of Parkinson's disease, and investigate the mechanism of the dysfunction of digestive system in Parkinson' s disease. Methods Substantia nigra dopaminergic neurons of the SD rats were damaged by injecting 6-hydroxydopamine (6-OHDA) in the brain substantia nigra. Western blotting analysis for the expression of NHERF family protein and CAL protein in the gastry, duodenum and colon of the control and 6-OHDA treated rats were performed. Results The Western blotting results showed that the protein level of PDZK1 and CAL in the gastric corpus was enhanced in 6-OHDA treated rats, the protein level of NHERF1, NHERF2 and PDZK1 in the duodenum was increased in 6-OHDA treated rats, the protein level of NHERF1, PDZK1 and CAL in the colon was enhanced in 6-OHDA treated rats. Conclusion The upregulation of NHERF family protein and CAL protein may contribute to the dysfunction of digestive system in 6-OHDA treated rats . These results provide a strong evidence for further studying the mechanisms of digestive system dysfunction in Parkinson' s disease.%目的 研究NHERF家族蛋白和CAL蛋白在帕金森病(Parkinson's disease,PD)消化系统中的表达,探讨帕金森病消化系统功能紊乱的可能机制.方法 用6-羟多巴(6-hydroxydopamine,6-OHDA)损毁大鼠中枢黑质多巴胺能神经元,建立帕金森大鼠模型.采用蛋白免疫印迹法(Western blotting),检测NHERF1、NHERF2、NHERF3、NHERF4以及CAL在6-OHDA处理模型大鼠胃体、十二指肠、结肠中表达的情况.结果 成功造模后,利用Western blotting的方法,发现帕金森模型大鼠胃体PDZK1和CAL蛋白表达水平上调,帕金森模型大鼠十二指肠NHERF1、NHERF2、PDZK1表达水平高于正常组,以及帕金森模型大鼠结肠NHERF1、PDZK1、CAL表达水平也高于正常组.结论 帕金森大鼠消化道组织中NHERF家族和CAL蛋白表达的上调,为研究帕金森患者胃肠道功能紊乱方面的机制提供线索.