碱性成纤维细胞生长因子对血管性痴呆大鼠海马胆碱能神经元的影响

摘要

目的 皮下注射碱性成纤维细胞生长因子(bFGF)于血管性痴呆大鼠,研究用药前后大鼠海马胆碱能神经元的变化.方法 制作血管性痴呆(VD)大鼠模型,随机取用VD大鼠模型12只,分治疗组6只,痴呆组6只.另外,取假手术组6只.皮下注射bFGF于治疗组中血管性痴呆大鼠.治疗5周后,以Morris水迷宫定位航行试验和空间探索试验来检测大鼠的学习记忆能力,乙酰胆碱转移酶(ChAT)免疫组织化学染色观察海马CA1区胆碱能神经元数目的 变化.结果 治疗组大鼠海马CA1区胆碱能神经元数目较痴呆组明显增多.结论 皮下注射bFGF后能迁移至海马,诱导海马产生具有ChAT活性神经元,所产生的ChAT活性神经元可能就是胆碱能神经元.%Objective To study the change of cholinergic neurons of hippocampus of vascular dementia rats after hypodermic injection of Basic Fibroblast Growth Factor ( bFGF ). Methods After the vascular dementia model was reproduced, the 12 model rats were randomly divided into therapy and dementia group. Another 6 rats in pseudo-operative group. The vascular dementia rats in therapy group were treated with hypodermic injection of bFGF. After 5 weeks, abilities of learning and memory of three section rats were tested by using the Morris water maze and space experimenty. Immunochemistry staining was used to observe the number of Choline acetyltransferase( ChAT ) positive neurons in the CAI subfield of the hippocampus. Result The number of the ChAT positive neurons in the CA1 subfield of the hippocampus were more significantly increased in therapy group than that in dementia group. Conclusion bFGF by hypodermic injection can migrate to hippocampus area. ChAT positive neurons can be found in hippocampus area. The ChAT positive neurons seem to be cholinergic neurons.