外源EGFP基因在肿瘤细胞中的稳定表达

摘要

The enhanced green fluorescent protein (EGFP) gene was transfected into the chromosomes of the human lung adenocarci-noma cells (A549) to construct the cell model with the EGFP for anti-cancer drugs screening in vitro, The application value of the model through studying in vivo as well as in vitro was studied. By electroporation, the EGFP gene was transfected into A549 cells and then by means of the G418 and gradient method, the high EGFP-expression cells that cultured for expansion were filtered out. The anticancer cells with fluorescence were obtained.%通过将增强型绿色荧光蛋白(EGFP)基因转染到人肺腺癌细胞(A549)内,建立以EGFP为探针的体外抗癌药物细胞株.使用电穿孔法导入EGFP基因到A549细胞;然后用G418筛选以及梯度稀释法筛选出EGFP高度表达的细胞株.初步建立稳定表达绿色荧光蛋白的人肺癌细胞株.