Post-translational modification regulates the activity of most eukaryote proteins. Deciphering of these post-translational modifications is indispensable insight into biological function. Histones with kinds of small basic proteins being subjected to various modifications especially at N-terninal tail are the best-studied proteins in post-translational modification research. The combination of these modifications constitutes the "histone code". Histone codes influence the chromatin structure modulating gene expression in development , growth, differentiation and homeostasis of cell. The investigation of modified histones can be used as a model to decipher complex combinational modifications and to analyze their molecular functions. The progress of analytical techniques, however, is essential to decipher the histone code. In this review, the application and progress of techniques applied in histone modification analysis were discussed.%翻译后修饰调控着真核生物大部分蛋白质的活性,这些修饰的解读对研究生物功能是必不可少的.组蛋白翻译后修饰是蛋白质翻译后修饰中研究的较好一类小分子碱性蛋白,易被各种生物大分子修饰,尤其易发生在N-末端的尾部.不同组合式修饰构成了"组蛋白密码",在细胞的发育、生长、分化和动态平衡中,组蛋白密码影响着染色体的结构状态,进而调控基因的表达状态.组蛋白翻译后修饰的研究可作为一种模式来解析蛋白质复杂的修饰状态及研究其分子功能.翻译后修饰分析技术的发展对组蛋白密码的解析是至关重要的.重点讨论组蛋白修饰分析技术的发展和应用.
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