Objective To determine the crocins in the fruits of Gardenia jasminoides. Methods The chromatographic condition was as follows: HPLC procedure was performed on the chromatographic column of Dikma Diamonsil C18 (4.6 mm ×250 mm, 5 μm) at 30 ℃, and the mobile phase was 0.15% formic acid solution-methanol in gradient elution. The flow velocity was 0. 6 mL/min and the detection wavelength was 440 nm. The mass spectrometry condition was as follows; source of electrospray ionization (ESI) , detection of negative ion mode and scan rang being m/z = 100 - 1400. The structure of crocins was determined combining the analysis on characteristics of ultraviolet-visible absorption spectra and MS/MS dissociation. Results There were 31 crocins identified from the fruits of Gardenia jasminoides by using HPLC-DAD-MS/MS, and 23 chemical constituents deduced according to the laws of MS/MS dissociation. Conclusion HPLC-DAD-MS/MS can be used for rapid screening and identifying crocins from the fruits of Gardenia jasminoides.%目的 鉴定栀子中藏红花素类色素成分.方法 色谱条件:Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm)；0.15％甲酸水-甲醇梯度洗脱,流速0.6 mL/min,柱温30℃,检测波长440 nm.质谱条件:电喷雾离子源(ESI)；负离子模式检测；扫描范围m/z 100-1400.结合化学成分的紫外-可见吸收光谱特征,与多级质谱(MS/MS)裂解特征分析,对其结构进行确证.结果 采用高效液相色谱-二级管阵列检测器(HPLC-DAD)鉴别了栀子中的31个藏红花素类色素成分,并根据MS/MS裂解规律推断了其中的23个化学成分.结论 HPLC-DAD-MS/MS可用于快速筛选和鉴定栀子中的藏红花素类色素成分.
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