为了实现心血管疾病的早期诊断,减少死亡率,建立一种基于近红外荧光免疫层析技术的肌酸激酶同工酶CK-MB快速检测技术.该检测方法使用激发光和发射光分别为777、792 nm的Dylight800作为荧光标记物,通过测量T线峰面积与C线峰面积的比值计算样品中CK-MB的浓度,实现了低成本、快速定量检测.通过25例临床标本检测并将结果与生化仪检测结果进行比对发现,本研究中建立的方法具有良好的相关性,R2=0.98.建立的CK-MB检测试剂可以满足急诊病人急性心肌梗死(AMI)快速检测的需求.%Early diagnosis of acute myocardial infarction ( AMI ) is critical to saving lives. Creatine kinase isoenzyme ( CK-MB) is an important biomarker for detecting AMI. A rapid detection technology was established based on near infrared fluorescence lateral chromatography. Dylight 800 with 777 and 792 nm excitation light and fluorescence was used as the fluorescence marker. The concentration of CK-MB in the sample can be accurately calculated by measuring the ratio of the peak area of the T-line to the C-line. The CK-MB test method established in this study has the characteristics of low cost, fast and accurate quantification. The results of 25 cases of clinical specimens and Beckman biochemical analysis were compared, the two detection methods have a good correlation, R2 =0. 98. The CK-MB test reagent established in this study can meet the rapid detection of AMI in emergency patients.
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