Creatine kinase isoenzyme electrophoretic technique and improved creatine kinase isoenzyme reagent for use therein

摘要

A creatine kinase reagent of the type comprising a buffer, creatine phosphate, adenosine diphosphate, D-glucose, hexokinase, glucose- 6- phosphate dehydrogenase, a thiol compound, magnesium ion, adenosine monophosphate, and a coenzyme selected from a group consisting of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and mixtures thereof. The reagent is characterized in that it further comprises an effective amount of an enzyme selected from a group consisting of phosphogluconate dehydrogenase and phosphogluconate dehydrogenase (decarboxylating).PPAn electrophoretic technique for assaying the relative distribution of creatine kinase characterized in that the above reagent is employed therein.