FHIT和MMP－2在喉癌进展过程中的表达及意义

摘要

目的：研究喉癌组织中脆性组氨酸三联体基因（fragile histidine traid ，FHIT）和基质金属蛋白酶－2（matrix metalloproteinase－2，MMP－2）的表达以及与临床病理特征的关系。方法：采用免疫组化链酶卵白素－生物素复合体（strep avidin－biotin complex ，SABC）法检测FHIT、MMP－2蛋白在92例喉鳞状细胞癌及相距10 mm癌旁组织和25例正常喉腔黏膜中的表达。结果：FHIT蛋白在喉癌组织中的阳性率（43．48％）明显低于癌旁组织（86．96％）及喉正常黏膜组织（92．00％），组间比较，差异均有统计学意义（ P ＜0．05），FHIT蛋白的表达与临床分期和淋巴结转移有关，而与年龄、分化程度无关；M M P－2在喉癌组织中的阳性率（79．35％）明显高于癌旁组织（18．48％）及喉正常黏膜组织（12．00％），经比较，差异均有统计学意义（ P ＜0．05），M M P－2的表达与分化程度和临床分期有关，而与年龄、淋巴结转移无关；此外，FHIT与MMP－2表达呈负相关。结论：FHIT、MMP－2的异常表达与喉癌的发生、发展及预后有关，二者在喉癌的发生、发展中起协同作用。%Objective :To explore and analyze the expression of fragile histidine traid (FHIT )and matrix metalloprotei_nase 2(MMP -2) protein in laryngocarcinoma and their relationship with clinicopathological .Methods :Expression of FHIT and MMP-2 protein was measured with immunohistochemistry method in 92 cases of laryngeal squamous cell car_cinoma(LSCC) tissue ,adjacent mucosa tissue which were 1 .0 cm away from carcinoma and 25 cases of normal laryngeal mucosa .Results :The expression of FHIT positive in laryngocarcinoma was 43 .48% significantly less than that adjacent to carcinoma(86 .96% ) and normal laryngeal mucosa (92% ) .There was a significant difference ( P<0 .05) .FHIT expres_sion level was correlated with clinical stages and lymphnode metastasis ,and has no correlation with age and differentiation level .The expression of MMP-2 positive in laryngocarcinoma was 79 .35% significantly higher than that adjacent to car_cinoma (18 .48% ) and normal laryngeal mucosa (12% ) .There was a significant difference ( P <0 .05) .MMP -2 ex_pression level was correlated with clinical stages and differentiation level ,has no correlation with age and lymphnode me_tastasis ;FHIT was negatively correlated with MMP -2 .Conclusion:Experimental results show that the abnormal ex_pression of FHIT and MMP-2 may play an important role in the occurrence ,development and prognosis of laryngocarci_noma .They may play a synergistic role in laryngeal cancer occurrence and development .