缺血性脑卒中患者的 IL-18表达水平及其基因105 A／C 多态性与肺炎衣原体感染的相关性研究

摘要

目的：探讨缺血性脑卒中患者体内IL-18表达水平的变化及其基因105 A／C多态性与缺血性脑卒中疾病的相关性。方法选择55例缺血性脑卒中患者为实验组，55例健康体检人群为对照组，收集全血标本后用ELISA检测试剂盒分别测定标本的血浆中CpnIgA、CpnIgG抗体水平及IL-18表达水平。应用聚合酶链反应-限制性片段长度多态性（ PCR-RFLP）技术对IL-18基因105位点进行基因分型。结果 AIS组CpnIgA阳性率明显高于健康对照组（χ2＝18．18，P＜0．05），而CpnIgG阳性率二者无显著性差异。 AIS组的IL-18表达水平明显高于健康对照组，且CpnIgA阳性的AIS患者的IL-18表达水平高于CpnIgA阴性患者，但其基因105 A／C基因多态性比较无统计学意义。结论缺血性脑卒中的发生、发展与肺炎衣原体慢性感染和IL-18水平有关，和IL-18的105 A／C基因多态性无明显相关，但具体作用机制还有待进一步研究。%Objective To explore the correlation of the infection of Chlamydia pneumoniae and the expression levels of interleukin-18 and its gene Polymorphisms in acute ischemic stroke (AIS) patients.Methods Detect the level of inter-leukin-18 and Chlamydia pneumoniae antibody CpnlgA and CpnIgG by ELISA in the plasma of the healthy control and AIS patients,then investgate interleukin-18 Gene 105A/C Polymorphisms by the method of restriction fragment length polymor-phism ( RFLP-PCR) .Results The positivity of CpnlgA in patients with AIS was higher than that in healthy control (χ2 =18.18).There was no difference in IgG positivity .The level of Interleukin-18 in the acute ischemic stroke patients was higher than that in healthy controls ,and which in the AIS patients with CpnlgA positivity was higher than that with CpnlgA negative.There was no significant difference in the interleukin-18 Gene 105A/C Polymorphisms.Conclusion The chronic infection of Chlamydia pneumoniae and the expression levels of Interleukin -18 was related to AIS and might involve in the trigger and development of AIS ,but there was no significant correlation between IL-18 gene105 A/C polymorphism with AIS .