乌桕根皮乙酸乙酯部位抑菌活性研究

摘要

[Objective] The antibacterial activity of acetate ethyl in the root bark of Chinese sapium was investigated. [Method] The antibacterial activity of the different flowing products of ethyl acetate after over normal phase silica gel column to pathogenic Escherickia coli ,Staphybcoccus aureus and Bacillus aeruginosus and Bacillus subtilis was determined with the disk diffusion method. [ Results] The results showed that the material with the antibacterial activity in sapium root bark primarily was in the part of large polarity,but there was antibacterial activity in the material of the methanol elution with larger polarity; and the inhibitory components of the soluble material of ethyl acetate was complex and the different parts had the variable inhibition to different bacteria and in general these materials had a certain inhibition to Staphylococcus aureus and Esche-richia coli. [ Conclusion] There was a quite difference in the antibacterial activity of different flowing products of ethyl acetate isolated by normal phase silica gel column chromatography.%[目的]研究乌桕根皮乙酸乙酯部位的抑菌活性.[方法]采用纸片扩散法,以病原菌大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)和绿脓杆菌(Bacillus aeruginosus) 以及枯草芽孢杆菌(Bacillus subtilis)作为受试菌,测定乌桕根皮乙酸乙酯部位过正相硅胶柱后各流分的抑菌活性.[结果]乌桕根抑菌活性物质主要存在极性较大的部分,但极性很大的甲醇洗脱部分也没有抑菌活性;乙酸乙酯可溶部抑菌成分较复杂,不同部分对不同细菌的抑制作用不同,总体来说对金黄色葡萄球菌和大肠杆菌有一定的抑制作用.[结论]乙酸乙酯部位经正相硅胶柱层析分离所得不同流分对各细菌的抑菌活性有很大区别.