[ Objective ] The study aimed to discuss the optimum technology condition for the ultrasonic microwave assisted extraction of poly-saccharides from Inonotus obliquus. [ Method] With water as the extracting solvent,the polysaccharides from I. obliquus were extracted by ultrasonic microwave assisted extraction technology. The effects of microwave power,microwave treating time and material-liquid ratio on the yield and purity of polysaccharides from I. obliquus were investigated through the response surface analysis. The technology parameters for extracting polysaccharides from I. obliquus by ultrasonic microwave assisted extraction were optimized and they were compared with that by traditional hot water extraction. [ Result ] The optimum technology condition for ultrasonic microwave assisted extraction of polysaccharides from I. obliquus was determined as follows:the extraction time ranged from 18.45 min to 24.50 min,the material-liquid was 1=20 and the microwave power ranged from 88. 3 W to 96. 7 W. Compared with the traditional hot water extraction,the ultrasonic microwave assisted extraction could shorten the extraction time greatly and increase the yield from 2.12% to 3. 25% as well as the purity from 64.03% to 73. 16% . [ Conclusion ] Compared with the traditional hot water extraction, the ultrasonic microwave assisted extraction could not only shorten the extraction time greatly, but also increase the yield and purity of polysaccharides from I. obliquus.%[目的]探讨超声-微波辅助技术提取桦褐孔菌多糖的最佳工艺条件.[方法]以水作为提取溶剂,用超声-微波辅助提取桦褐孔菌多糖,通过响应面分析法考察微波功率、微波处理时间和料水比对桦褐孔菌多糖得率和纯度的影响,优化超声-微波提取桦褐孔菌多糖的工艺参数,并和传统水浴浸提法进行比较.[结果]超声-微波辅助技术提取桦褐孔菌多糖的最佳工艺条件为:提取时间18.45～24.50 min,料液比1∶ 20,微波功率88.3～96.7 W.与传统的水浴浸提法相比,超声-微波提取法可大大缩短提取时间,得率由2.12%增加到3.25%,纯度由64.03%增加到73.16%.[结论]与传统的水浴浸提法相比,超声-微波提取法不仅缩短了提取时间,而且提高了桦褐孔菌多糖的得率和纯度.
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