[Objective] The study aimed to conduct the germplasm resource conservation and large-scale seedling propagation on the wild cassava. [ Method] The tissue culture tnelhod was use lo make for the rapid propagation. [ Result] The medium suitable for the callus induction and the adventitious bud differentiation of the wild cassava was MS +6-BA 0. 10-0.50 mg/L + NAA 0. 1 -0.5 mg/L; the medium suitable for the proliferation was MS (Fe content doubling) +6-BA0. 50 -0.65 mg/L + IBA0. 1 mg/L + PVP 200 mg/L; the medium suitable for rooting was MS (Fe content doubling) +0. 1% AC and the rooting rate was more than 90% ; when the test tube seedlings was made for the domestication in the matrix with the ratio of the crud river sand to the fine river sand of 2:1, the survival rate was 85%. [ Conclusion] The rapid propagation method for the wild cassava was established.%[目的] 对野生木薯进行种质资源保存和大规模种苗繁殖.[方法] 采用组织培养的方法进行快速繁殖.[结果] 适宜野生木薯愈伤与不定芽分化的培养基为MS +6-BA 0.10～0.50 mg/L+ NAA 0.1～0.5 mg/L；继代增殖适宜培养基为MS(Fe含量加倍)+6-BA0.50～0.65 mg/L+ IBA 0.1 mg/L+ PVP 200 mg/L;适宜生根的培养基为MS(Fe含量加倍)+0.1％ AC,生根率达90％以上；在粗河沙∶细河沙=2∶1基质中驯苗,成活率可达85％以上.[结论] 建立了野生木薯的快速繁殖方法.
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