丹东野生香百合的组织培养和快繁技术研究

摘要

对丹东地区野生香百合的组织培养进行了研究。结果表明：以球根鳞片做外植体培养在MS＋BA 1．0 mg/L ＋ NAA 0．4 mg/L培养基上可诱导出小鳞茎，在MS＋BA 1 mg/L ＋ NAA 0．1 mg/L培养基上继代可增殖，增殖系数5倍以上；壮苗培养以MS＋BA 0．5 mg/L ＋ NAA 0．1 mg/L 为好；最适生根培养为1/2M S＋IBA 0．5 mg/L ，生根率达90％，；移栽基质以纯沙为最好，成活率达95％以上。%The rapid propagation of Lillum brownii was developed through culture of scales and stem seg-ments in vitro .The best induction medium for scales was MS+BA 1 .0 mg/L + NAA 0 .4 mg/L ;the best proliferation medium for shoots was MS +BA 1 mg/L + NAA 0 .1 mg/L .Propagation coefficient was 5 times .MS+BA 0 .5 mg/L + NAA 0 .1 mg/L on seedling cultivation as well .The best induction medium for roots was 1/2MS+IBA0 .5 mg/L .the rate was 90% .When micropropagated plantlets with well-developed root systems were transferred to planting bed of sand in a greenhouse ,95% of the plant-lets survived .