Process and nutrient medium for micropropagation of cassava

摘要

A medium is provided which is useful in a fast and convenient system for multiplying cassava plants vegetatively by means of in vitro micropropagation of nodal cultures. This medium includes in parts per million the following: ##TBL1## and suitable amounts of essential ingredients for a cassava micropropagation medium.PPIn paticular, the medium also contains in part per million about: 3.0 H.sub. 3 BO.sub.3, 10.0 MnSO.sub.4.H.sub.2 O, 6.0 ZnSO.sub.4.7H.sub.2 O, 0.25 Na. sub.2 MoO.sub.4.2H.sub.2 O, 0.025 CuSO.sub.4.5H.sub.2 O, 0.025 CaCl.sub. 2. 6H.sub.2 O, 0.75 KI, 2.5 Nicotinic acid, 10.0 Thiamine HCl, 1.0 Pyridoxine HCl, 100.0 M-inositol, 0.5 Glycine, 0.5 Folic acid, 0.05 Biotin, 0.5 D-Ca-Pantothenate, 0.25 Riboflavin, 0.5 Ascorbic acid, 0.1 choline chloride, 1.0 L-cysteine HCl, 10.0 Malic acid and 50.0 Casein Hydrolysate. Furthermore, the medium desirably contains about 3 percent sucrose or glucose.