黄粉虫酪氨酸羟化酶基因的克隆与序列分析

摘要

[目的]克隆鞘翅目昆虫黄粉酷氨酸羟化酶(TmTH)基因.[方法]采用cDNA末端快速扩增(RACE)技术和PCR方法克隆了鞘翅目昆虫黄粉虫TmTH基因,并对其进行了生物信息学分析、[结果]TmTH cDNA全长2103bp,包含1605bp的开放式阅读框,可编码534个氨基酸残基,蛋白质分子量为60.66kD,等电点为5.70,构建了其系统进化树.[结论]TmTH基因的克隆为鞘翅目昆虫酪氨酸羟化酶的研究提供了参考.%[ Objective ] The research aimed to clone tyrosine hydroxylase gene of Tenebrio molitor (TmTH) of Coleoptera insects. [ Method ]RACE and PCR methods of cDNA tail end was used to clone TmTH gene of Coleoptera insects, and bioinformatics analysis was carried out.[ Result] The overall length of TmTH cDNA was 2 103 bp, which including 1605 bp open reading frame (ORF), thus can encoding a protein of 534 amino acid residues, and the molecular weight and pI of TmTH were 60.66 kD and 5.70,respectively. Based on that, we constructed the phylogenetic tree of TmTH. [ Conclusion] Molecular cloning of the tyrosine hydroxylase gene in mealworm, Tenebrio molitor can provide reference for further research of tyrosine hydroxylase of Coleoptera insects.