[目的]研究3种抑制菌株代谢活性的长期保藏方法对冬虫夏草菌种保藏的效果.[方法]以菌丝体生长得率、虫草多糖、虫草酸作为衡量菌种的质量指标,采用低温冻结、液氮冷冻和冷冻干燥保藏等3种方法对C-03冬虫夏草菌种的保藏效果进行比较.[结果]真空冷冻干燥保藏,以脱脂牛奶为保护剂,4℃预冷30 min,然后于-20℃预冻2 h,再于-80℃预冻24 h的阶段降温条件下,菌株萌发长势、菌丝得率及有效成分含量优于其他2种保藏方法.[结论]真空冷冻干燥保藏为冬虫夏草菌种较为理想的长期保藏方法.%[ Objective ] To compare the preservation effects of three long-term preservation methods on inhibiting the metabolic activity of Cordyceps sinensis culture. [ Method ] With the growth of mycelium,polysaccharide of Cordyceps sinensis and cordycepic acid as indicies, the effects of cryogenic ,liquid nitrogen freezing and freeze-drying on conserving C. sinensis culture of C -03 were comparatively studied. [ Result ] The C.Sinensis culture through vacuum freeze-drying preservation due to skimmed milk and phased cooling of 4 ℃ for 30 min, - 20 ℃ and - 80 ℃freezing for 2 h and 24 h respectively was growing well when germination, the yield of mycelial growth and active ingredients were relatively higher than the other two methods. [ Conclusion] Vacuum freeze-drying preservation was the desirable method to preserve C. sinensis culture.
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