东北刺人参愈伤组织诱导和继代的研究

摘要

[目的]研究东北刺人参愈伤组织的诱导和继代方法.[方法]以取自长白山的刺人参植株为材料,研究不同外植体、培养基、激素对刺人参愈伤组织诱导的影响.[结果]叶片为刺人参组织培养的最适宜外植体；5月为外植体的最佳取材时期；以MS为基本培养基,并添加1.0 mg/L 2,4-D和1.0 mg/L 6-BA的培养基中愈伤组织诱导率最高；最佳继代培养基为MS+ 6-BA 2.0 mg/L+ NAA 0.2mg/L.[结论]东北刺人参的最适愈伤诱导培养基和继代培养基分别是:MS +2,4-D 1.0 mg/L +6-BA 1.0 mg/L和MS+ 6-BA 2.0 mg/L+ NAA 0.2 mg/L.%[Objective] To study the callus induction and subculture method of Oplopanax elaus. [Method] With 0. elaus as material, the effect of different explants, mediums and hormones on callus induction was studied. [ Result] The results showed that leaf was the best suitable organs for tissue culture. May was the best period to draw materials. MS added to 2.0 mg/L 2,4-D and 1. 0 mg/L 6-BA had high induction rate on callus; and the optimal subculture medium was MS + 6-BA 2.0 mg/L + NAA 0. 2 mg/L. [ Conclusion] The best callus induction medium and ubculture medium of 0. elaus: MS + 2,4-D 1.0 mg/L + 6-BA 1.0 mg/L and MS + 6-BA 2.0 mg/L + NAA 0. 2 mg/L.