红栓菌液态发酵后马钱子总碱中2种生物碱含量的变化

摘要

[Objective] To investigate the content change of strychnine and brucine after the liquid fermentation of SEMEN STRYCHNI alkaloids by Trametes cinnabarina (Jacq. ) Franeh. [Method] SEMEN STRYCHNI alkaloids were fermented by the T. cinnabarina under the optimal conditions. The contents of strychnine and brucine in broth were determined by ion pair RP-HPLC and the bioraass of mycelium of T. cinnabarina was detected. [Result] The average biomass of the mycelium could reach up to 9. 8 g/L, 25.6% more than that of the control. The total content of strychnine and brucine in the culture medium was 31.05% , 16.10% less than that of the control. [Conclusion] The alkaloids could promote the fungal growth and decrease the content of toxic constituents by liquid-state fermentation.%[目的]研究经红栓菌液态发酵后马钱子总碱中士的宁(Stry)和马钱子碱(Bru)的含量变化.[方法]以优化的红栓菌发酵工艺条件液态发酵马钱子总碱,采用反相离子对色谱法分析发酵液中Stry和Bru的含量,并测定红栓菌菌体的生物量.[结果]发酵组的菌体平均生物量为9.8g/L,较发酵空白组增加了25.6％；Stry和Bru的含量总和平均值为31.05 g/L,较空白对照组降低了16.10％.[结论]马钱子总碱对红栓菌菌丝体的生长有一定的促进作用,液态发酵培养可降低马钱子总碱中毒性成分的含量.