在单因素实验条件优化的基础上,采用L16(45)正交试验,以士的宁和马钱子碱的质量分数为主要考察指标,结合菌体生物量,研究不同碳源、氮源、装液量、中药量、菌球数量以及发酵时间对红栓菌发酵马钱子的影响,优化红栓菌液体发酵马钱子的最佳培养基和培养条件.优化工艺为:以麦芽糖为碳源,豆饼粉为氮源,装液量为100mL(250mL的三角瓶),加入0.25 g马钱子细粉,接入3个大小均一的红栓菌菌球,28℃摇床培养5d.与发酵空白比较,红栓菌—马钱子发酵液中士的宁和马钱子碱的质量分数总和降低了8.74％,菌体生物量达10.6 g/L,增加37.7％,同时马钱子碱氮氧化物和士的宁氮氧化物的质量分数增加.马钱子能促进红栓菌菌体的生长,液体发酵培养可达到降低马钱子中毒性成分质量分数的目的.%Based on the results of the single factor (carbon and nitrogen sources), the effect of broth content, mycelial pellet amount , powder weight and fermentation time on alkaloids content and biomass growth were further discussed by using L16 (45) orthogonal test. The optimized culture conditions were acquired and listed as follows: maltose is carbon source, soybean cake powder is nitrogen source and liquid volume of 100 mL (a 250 mL flask), by adding 0. 25 g powder, inoculated three evenly sized mycelial pellet, cultured at 28 degrees Celsius for 5 days. After optimization the total content of strychnine and brucine in the culture medium reduced 8. 74% compared with the control. Biomass could reach 10. 6 g/L. Its rising rate was 37. 7%. Meanwhile strychnine N-oxide and brucine N-oxide increased. The results showed the drug could promote the fungal growth and it can decrease the content of toxic constituents by liquid fermentation.
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